2026-10-13T15:05:53Z https://www.scidb.cn/oai

10.57760/sciencedb.40736 2026-06-23T11:15:16Z

2026-06-23 Small RNA sequencing and RNA sequencing of VACV-infected cells doi:10.57760/sciencedb.40736 en Libraries of small RNAs were constructed using TruSeq Small RNA Library Preparation Kits (Illumina) according to manufacturer’s protocols, and then sequenced by Illumina HiSeq 2000 at Bioacme (Wuhan, China). Quality control and adapter trimming were performed using Trim Galore (v0.6.4) with default parameters, and reads length between 17 and 70 were used for subsequent analyses. The sequenced reads were mapped to the human genome (release hg19) by using Bowite2 2.3.5.  Libraries of RNAs from RNA-IP samples were constructed using MGIEasy RNA Library Preparation Kits (BGI) according to manufacturer’s protocols, and then sequenced by MGISEQ2000 (BGI) at Wuhan Institute of Virology, CAS. Quality control and adapter trimming were performed using Trim Galore (v0.6.4) with default parameters. All clean reads were aligned to the viral genome using STAR (v2.7.11b) with strand-specific quantification, allowing separate abundance estimation for the (+)- and (-)-stranded genomes. Antiviral RNAi; poxvirus; vsiRNA Yang Qiu PUBLIC https://creativecommons.org/licenses/by-nc/4.0/ dataset Science Data Bank