2026-10-10T22:40:45Z https://www.scidb.cn/oai

10.57760/sciencedb.41434 2026-07-06T22:14:21Z

2026-07-06 SDRG data of Liu Li group doi:10.57760/sciencedb.41434 en 1. Nucleic Acid ElectrophoresisValidation of SDRG expression in the Drosophila head;Identification of the SDRG mutant.2. Quantitative PCR (qPCR)Detection of frq1 and frq2 RNA levels​ in the heads of wild-type and SDRG mutant flies;Detection of frq1 and frq2 RNA levels​ in the heads of flies following individual knockdown of candidate SDRG-binding protein-coding genes.3. Neuromuscular Junction (NMJ) ImmunohistochemistryPhenotypic analysis and quantification of NMJ synaptic terminals​ in third-instar larvae of wild-type and SDRG mutants;Phenotypic analysis and quantification of NMJ synaptic terminals​ in third-instar larvae following individual knockdown of candidate SDRG-binding protein-coding genes;Phenotypic analysis and quantification of NMJ synaptic terminals​ in third-instar larvae following individual overexpression of frq1and frq2;Genetic interaction​ analysis between SDRG and frq1/ frq2;Genetic interaction​ analysis between the candidate protein Cora and frq1/ frq2;Genetic interaction​ analysis between the candidate protein Hrb and frq1/ frq2.4. RNA Immunoprecipitation (RIP)Screening for interaction sites between Cora and SDRG​ following SDRG overexpression in the Drosophila nervous system;Screening for Cora binding sites on frq2 RNA​ in wild-type flies;Comparative analysis of the binding occupancy at positive Cora–frq2 RNA interaction sites​ between wild-type and SDRG mutant flies.5. RNA Pull-Down coupled with Mass SpectrometryScreening of SDRG-interacting proteins​ from Drosophila heads using biotin-labeled sense probes​ and cold competitor probes. SDRG; Drosophila; NMJ; frq1; frq2 李美霞 PUBLIC https://creativecommons.org/publicdomain/zero/1.0/ dataset http://www.doi.org/10.1096/fj.202601174R. Science Data Bank