2026-10-15T02:01:04Z https://www.scidb.cn/oai

10.57760/sciencedb.41966 2026-07-09T16:49:49Z

2026-07-09 Metabolomics of Liriomyza trifolii treated with bitter gourd leaf extract doi:10.57760/sciencedb.41966 en For metabolomics, an LC-MS/MS system (Waters Acquity I-Class PLUS UHPLC coupled with Xevo G2-XS QTof) equipped with an HSS T3 column (1.8 μm, 2.1×100 mm) was used. Mobile phases were 0.1% formic acid in water (A) and acetonitrile (B), with a 2 μL injection. MassLynx V4.2-controlled MSe mode acquired MS and MS/MS data at 0.2 s/scan (low/high collision energy: off/10–40 V). ESI parameters: capillary ±2500/–2000 V, cone 30 V, source/desolvation temps 100/500°C, cone/desolvation gas 50/800 L/h. Raw data were processed by Progenesis QI for peak picking, alignment, and identification against METLIN and an in-house library. After total peak area normalization, PCA and Spearman correlation assessed repeatability and QC. Identified compounds were annotated via KEGG, HMDB, and Lipidmaps. Differential metabolites were screened by FC > 2, P < 0.05, and VIP > 1 (OPLS-DA, ropls R package, 200 permutations). KEGG enrichment was tested by hypergeometric distribution. Metabolomics; Liriomyza trifolii ; Bitter gourd leaf extract Yawen Chang PUBLIC https://creativecommons.org/licenses/by/4.0/ dataset Science Data Bank