2026-10-14T00:51:21Z https://www.scidb.cn/oai

10.57760/sciencedb.42513 2026-07-13T09:48:22Z

2026-07-13 Mechanism of icariin in delaying skin photoaging based on network pharmacology and experimental verification – Raw data doi:10.57760/sciencedb.42513 en Abstract: Based on network pharmacology, molecular docking technology, and cell experiments, this study explores the role and potential mechanism of icariin (ICA) in delaying skin photoaging. Using databases such as SwissTarget Prediction and GeneCard to screen potential targets for ICA; Obtain skin photoaging related targets from databases such as OMIM and DrugBank, and import their shared targets into the STRING database for analysis. Construct a protein interaction network using Cytoscape 3.7.2 software. Further use the DAVID database for GO and KEGG enrichment analysis to identify their key signaling pathways. Verify the docking between ICA and core targets using AutoDock 4.2.6 software. Finally, the key targets were validated through cell experiments. According to network pharmacology analysis, there are a total of 123 potential targets associated with ICA and skin photoaging, including 10 key core targets, including B-cell lymphoma-2 (BCL-2) and cysteine aspartate specific protease-3 (Caspase-3). GO and KEGG enrichment analysis showed that these targets mainly involve signaling pathways such as phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT). The molecular docking results showed that ICA has strong binding activity with target BCL-2 and Caspase-3. The cell experiment results showed that ICA can significantly inhibit the aging and apoptosis of HaCaT cells. In addition, compared with the ultraviolet B (UVB) group, the ICA group showed a decrease in the expression levels of BCL-2-associated X protein (BAX) and cleaved Caspase-3 protein, while the expression levels of phosphorylated PI3K (p-PI3K), phosphorylated AKT (p-AKT), and BCL-2 protein were significantly increased. In summary, ICA inhibits cell apoptosis and delays skin photoaging by regulating the PI3K/AKT signaling pathway.  icariin; skin photoaging; PI3K/AKT; Skin photoaging; Network pharmacology; Molecular docking; Mechanism of action PI3K/AKT Hou Li Liu Cuihua RESTRICTED dataset Science Data Bank