2026-10-14T12:43:37Z https://www.scidb.cn/oai

10.57760/sciencedb.43243 2026-07-16T15:58:24Z

2026-07-16 Microbial community assembly in rapeseed under uranium stress: 16S rRNA sequencing across soil-root-leaf niches doi:10.57760/sciencedb.43243 en This dataset comprises 16S rRNA gene amplicon sequencing data generated from a pot experiment investigating microbial community dynamics under uranium stress in rapeseed (Brassica napus). Soil samples were collected from three treatments: original soil, sterilized soil, and sterilized-inoculated soil, with or without 150 mg kg⁻¹ uranium addition. Four ecological niches were sampled: bulk soil, rhizosphere soil, root endosphere, and leaf phyllosphere. The V3–V4 region of the 16S rRNA gene was amplified using primers 338F/806R and sequenced on the Illumina MiSeq platform (2×300 bp paired-end). Raw reads were quality-filtered, denoised, and merged using the DADA2 pipeline, and operational taxonomic units (OTUs) were clustered at 97% similarity. Taxonomic assignment was performed against the Greengenes 13.8 database. Quality control included negative controls (extraction blanks and PCR negatives) and rarefaction to 95% of the minimum sample depth to ensure comparability. These data are valuable for studying microbial community assembly, niche differentiation, functional redundancy/compensation, and plant–microbe interactions in uranium-contaminated soils, and can support comparative analyses with other heavy-metal or phytoremediation studies. 16S rRNA; Uranium; microbiota 郭堤 PUBLIC https://creativecommons.org/publicdomain/zero/1.0/ dataset http://www.doi.org/10.3389/fmicb.2026.1860545 Science Data Bank