2026-10-11T23:27:10Z https://www.scidb.cn/oai

10.57760/sciencedb.43649 2026-07-21T16:10:16Z

2026-07-21 Isolation of Marine-Derived microorganisms for PET Biodegradation doi:10.57760/sciencedb.43649 en The long-term accumulation of polyethylene terephthalate (PET) in marine environments may drive the evolution of microbial degradation capabilities, positioning the ocean as a valuable reservoir for discovering novel PET-degrading microorganisms. In this study, we isolated 305 candidate PET-degrading microorganisms from marine samples using PET powder as the sole carbon source. Subsequent evaluation of degradation performance via scanning electron microscopy and fourier-transform infrared spectroscopy analysis identified 14 isolates capable of degrading PET film. These 14 strains belonged to 14 distinct species, none of which, to the best of our knowledge, have been previously documented as PET degraders. Among them, Microbacterium aurum SCSIO 85700 exhibited the most potent PET-degrading activity, achieving a weight loss of 2.1 mg and a 6.5% increase in relative crystallinity over 30 days. Genome analysis revealed the genetic basis underlying PET degradation and associated metabolic pathways in strain SCSIO 85700. Notably, genome mining and structural modeling identified two candidate polyester hydrolases, MA2267 and MA2443, possessing conserved His–Asp–Ser catalytic triads and exposed substrate-binding clefts resembling those of characterized PET-degrading enzymes, suggesting their potential involvement in PET depolymerization. Collectively, this study expands the recognized diversity of marine PET-degrading microorganisms and provides microbial resources for sustainable PET bioremediation. polyethylene terephthalate; marine environments; plastic biodegradation 李青连 田新朋 RESTRICTED dataset Science Data Bank