2026-10-13T23:00:06Z https://www.scidb.cn/oai

10.57760/sciencedb.43909 2026-07-23T15:18:25Z

2026-07-23 16S rRNA gene high-throughput sequencing data of rhizosphere microorganisms of broad bean grown at different altitudes in Qinghai Province doi:10.57760/sciencedb.43909 en Soil total DNA was extracted using the FastDNA® Spin Kit for Soil. The V4 region of the bacterial 16S rRNA gene was selected as the amplification target, and PCR amplification was performed with universal primers 515F (5′-GTGCCAGCMGCCGCGGTAA-3′) and 806R (5′-GGACTACHVGGGTATCTAAT-3′). Pro Taq DNA polymerase was used for PCR reactions, with a total reaction volume of 20 μL. The PCR amplification program was set as follows: initial denaturation at 95 °C for 3 min; followed by 27 cycles of denaturation at 95 °C for 30 s, annealing at 55 °C for 30 s, and extension at 72 °C for 45 s; and a final extension at 72 °C for 10 min. After quality verification of the amplified products, high-throughput sequencing was conducted on the Illumina MiSeq PE300 platform by Shanghai Majorbio Bio-pharm Technology Co., Ltd. Faba bean; rhizosphere microorganisms; community structure; community stability; ecological niche 颜珲璘 PUBLIC https://creativecommons.org/licenses/by-nc-nd/4.0/ dataset Science Data Bank