2026-10-14T14:21:45Z https://www.scidb.cn/oai

10.57760/sciencedb.44189 2026-08-17T09:51:03Z

2026-08-17 Raw data for functional characterization of the SvMDR1 gene in Stemphylium vesicarium doi:10.57760/sciencedb.44189 en This dataset contains the complete raw experimental data for the functional characterization of the SvMDR1 gene in Stemphylium vesicarium. The data were collected from wild-type (WT), ΔSvmdr1 knockout, complemented (ΔSvmdr1-Com), and overexpression (OE-Svmdr1) strains, covering the following phenotypic assays:Colony radial growth rate (on PDA at 28°C for 7 days)Stress tolerance (growth inhibition rates under H₂O₂, CoCl₂, and ethanol stress)qRT-PCR gene expression (relative expression of SvMDR1, SvPKS1, and other genes)qRT-PCR validation of RNA-seq DEGs (verification of differentially expressed genes identified by transcriptome analysis)Altersolanol A production (quantified by HPLC)Virulence assay (lesion areas on detached tomato leaves)Statistical calculations (including t-test results, Spearman correlation coefficients, and linear regression outputs for all figures)All data were obtained from three independent biological replicates and are provided in Excel format. This dataset is associated with the research paper entitled "The vesicle-localized oxidoreductase SvMDR1 promotes Altersolanol A biosynthesis and virulence in Stemphylium vesicarium" Stemphylium vesicarium; SvMDR1; gene knockout; pathogenicity; Altersolanol A; stress tolerance; transcriptome validation; qRT-PCR Zhou Qian Zhu Hongjian Cao Junjie PUBLIC https://creativecommons.org/licenses/by/4.0/ dataset Science Data Bank