2026-10-14T23:40:19Z https://www.scidb.cn/oai

10.57760/sciencedb.44492 2026-07-29T16:59:15Z

2026-07-29 Determination of coumarin via a two-step chromogenic reaction coupled with capillary electrophoresis doi:10.57760/sciencedb.44492 en In this study, we optimized the chromogenic reaction of coumarin by utilizing borate as a chelating agent. Our result showed that alkaline hydrolysis of coumarin at pH 12.1 produced the strongest color response in the presence of 8.3 mM borate. In the chromogenic phase, after adjusting the pH to 8.8 with boric acid, the reaction efficiency remained high, and the stability of the colored product was superior compared to other pH conditions. We further established a capillary electrophoresis (CE) analytical condition for the colored product. This method exhibited a strong linear correlation within the coumarin concentration range of 10-200 mg L–1. Using this method, coumarin concentrations in Medicago sativa and Trifolium pratense seeds were successfully determined.  alkaline condition; coumarin; color reaction; capillary electrophoresis; Gibbs reagent 张泰劼 郭文磊 张纯 EMBARGO https://creativecommons.org/licenses/by/4.0/ dataset Science Data Bank