2026-10-13T15:23:19Z https://www.scidb.cn/oai

10.57760/sciencedb.44503 2026-07-30T11:08:57Z

2026-07-30 Single-nucleus transcriptome datasets of the ligular region at preligule band and ligule outgrowth stages in 12-day-old maize B73 seedlings doi:10.57760/sciencedb.44503 en This dataset contains single‑nucleus RNA sequencing (snRNA‑seq) data from maize ligular regions at two developmental stages: the preligule band stage (S1) from the fourth‑leaf ligular region and the ligule outgrowth stage (S2) from the third‑leaf ligular region. For each stage, tissues were dissected from 30 seedlings, and nuclei were isolated using a modified Galbraith buffer‑based protocol. The crude nuclear suspension was purified by fluorescence‑activated cell sorting (FACS) to enrich intact nuclei. Approximately 20,000 purified nuclei from each sample were loaded onto the 10x Genomics Chromium Controller, and libraries were constructed using the Chromium Next GEM Single Cell 3' Kit v3.1. Sequencing was performed on an Illumina NovaSeq 6000 platform with 150‑bp paired‑end reads. This dataset enables high‑resolution transcriptomic analysis of ligule development, providing a valuable resource for studying cell‑type‑specific gene expression dynamics in maize. sn-RNA-seq; ligule; maize Fang Wei Xuefei Yang Guiling Sun EMBARGO https://creativecommons.org/licenses/by/4.0/ dataset Science Data Bank