2026-10-14T20:37:51Z https://www.scidb.cn/oai

10.57760/sciencedb.44788 2026-07-30T11:08:40Z

2026-07-30 A rapid diagnostic kit for monitoring insecticide phenotypic susceptibility in the cotton bollworm Helicoverpa armigera (Lepidoptera: Noctuidae) doi:10.57760/sciencedb.44788 en Helicoverpa armigera (Lepidoptera: Noctuidae) is a globally significant agricultural pest with documented resistance to various insecticides. Rapid and accurate diagnostic tools for assessing insecticide susceptibility are urgently needed to enhance the efficiency of pest control for farmers. The modified centrifuge tube bioassay diagnostic kit provides detection results within 3 h, identifying the diagnostic doses as 5087.32 ng/cm² for indoxacarb, 300.35 ng/cm² for chlorpyrifos, and 461.79 ng/cm² for chlorantraniliprole. A regression equation was established to correlate mortalities at diagnostic doses with actual resistance ratios in various populations of H. armigera using the leaf-dipping method. The diagnostic mortality of H. armigera showed a negative correlation with actual resistance ratios to indoxacarb (r = -0.9674, p = 0.0070), chlorpyrifos (r = -0.8474, p = 0.0696), and chlorantraniliprole (r =-0.8833, p = 0.0470). Furthermore, the validation of kit accuracy was applied to field populations in Handan region of China, selected from the years 2017, 2018, and 2019. The datasets of chlorpyrifos and chlorantraniliprole displayed a strong correlation (r = 0.9968, P < 0.001). Nevertheless, the diagnostic dose of LD90 failed to promptly reveal the resistance level to indoxacarb in the Handan regions (ranging from 19.25- to 29.48 fold), suggesting that these kits are constrained by the detection threshold of the equation. In conclusion, a diagnostic kit based on has been developed for the rapid and accurate detection of susceptibility to these three insecticides in H. armigera. This insecticide diagnostic kit for H. armigera serves as a preliminary, accessible and cost-effective screening tool for identifying effective insecticides. Leaf-dipping method; The diagnostic dose; Centrifuge tube bioassay Wang qinqin PUBLIC https://creativecommons.org/licenses/by-nc-nd/4.0/ dataset Science Data Bank