2026-10-11T02:31:28Z https://www.scidb.cn/oai

10.57760/sciencedb.45004 2026-08-04T17:28:48Z

2026-08-04 Physiological dataset of Calamagrostis angustifolia under 14 years of nitrogen addition treatment doi:10.57760/sciencedb.45004 en This dataset is generated based on the long-term nitrogen addition simulation experimental platform at the Sanjiang Plain Wetland Ecological Positioning Research Station (47°45′39″ N, 133°37′04″ E). The experiment began in May 2010, covering a spatial area of nine 20 m × 20 m plots. Three treatment levels were established: control (0 g N·m⁻²·a⁻¹), low nitrogen (4 g N·m⁻²·a⁻¹), and high nitrogen (8 g N·m⁻²·a⁻¹). Ammonium nitrate solution is sprayed evenly in mid-May (the green-up period of Calamagrostis angustifolia) each year. Sampling and measurements for this dataset were conducted in mid-July 2024 (i.e., after 14 consecutive years of nitrogen addition). A cross-plot random sampling method was adopted spatially, with five 1 m × 1 m quadrats randomly established within the three spatial replicate plots of each nitrogen treatment.Leaf photosynthetic physiological traits (net photosynthetic rate, stomatal conductance, intercellular CO₂ concentration, and transpiration rate) were measured directly in vivo using an LI-6400XT portable photosynthesis system between 9:00 and 11:00 on clear days. During the measurements, the photosynthetically active radiation (PAR) was strictly controlled at 1500 μmol·m⁻²·s⁻¹, the ambient CO₂ concentration was maintained at 420 μmol·mol⁻¹, and the leaf temperature was stabilized at 28.0±0.1 °C. Leaf area was measured using an LI-3000c portable leaf area meter, and leaf fresh weight and oven-dried dry weight were weighed using an electronic balance with a precision of 0.0001 g. Regarding ecological stoichiometric characteristics, leaf carbon and nitrogen contents were determined using a Vario Max Cube elemental analyzer, while leaf phosphorus content was determined using sulfuric acid-hydrogen peroxide digestion followed by molybdenum-antimony anti-spectrophotometry. Derived functional traits (such as water use efficiency, stomatal limitation value, specific leaf area, and photosynthetic nitrogen use efficiency), as well as the phenotypic plasticity index (PPI) and phenotypic integration (PI), were all calculated based on the measured basic data.This dataset is presented as a general tabular file. The data table contains systematic observation records from 45 quadrats. The row labels represent different sample IDs and their corresponding spatial plots and nitrogen treatment groups. The column labels include 14 leaf functional trait indicators and integration indices, with their corresponding units as follows: net photosynthetic rate (μmol·m⁻²·s⁻¹), stomatal conductance (mol·m⁻²·s⁻¹), transpiration rate (mmol·m⁻²·s⁻¹), intercellular CO₂ concentration (μmol·mol⁻¹), water use efficiency (μmol·mmol⁻¹), stomatal limitation value (dimensionless), carbon content (mg·g⁻¹), nitrogen content (mg·g⁻¹), phosphorus content (mg·g⁻¹), C:N ratio, C:P ratio, N:P ratio, specific leaf area (cm²·g⁻¹), leaf area (cm²), leaf dry mass (g), leaf dry matter content (%), and photosynthetic nitrogen use efficiency (μmol·g⁻¹·s⁻¹). Calamagrostis angustifolia; nitrogen addition; physiological traits chen ming yi PUBLIC https://creativecommons.org/licenses/by/4.0/ dataset Science Data Bank