2026-10-13T21:30:16Z https://www.scidb.cn/oai

10.57760/sciencedb.cjb.00014 2026-08-28T10:29:13Z

2026-08-28 Transcriptome data of potato Stas2-15 gene overexpression doi:10.57760/sciencedb.cjb.00014 en 1. Data collection sample source The material used in the research institute is tetraploid potato Dixirui (DES) provided by the Potato Molecular Breeding Laboratory of the College of Agriculture, Shanxi Agricultural University; Our research group has previously cloned the Stas2-15 gene, constructed a potato Stas2-15 overexpression vector, and obtained a potato Stas2-15 gene overexpression positive strain (OE-2). Six samples were obtained by collecting aboveground tissues from normal growing Dixirui (DES) and transgenic plants (OE-2), with three replicates per plant. After sampling, immediately freeze and store the sample in liquid nitrogen. 2. Data processing methods (1) Bioinformatics analysis A phylogenetic tree was constructed using MEGA11 (Molecular Evolutionary Genetics Analysis Version 11) software to analyze the amino acid sequences of as2-15 from 19 different species, including sweet potato, tomato, scopolamine, tobacco, and aloe vera. The amino acid sequences of As2s proteins from 19 plants were paired in multiple ratios using DNAMAN. EXPASY analysis of physicochemical properties of Stas2-15 protein（ https://web.expasy.org/protscale/ ）Using the web website Protparam（ http://www.expasy.org/Profiles/Protparam.html ）Statistically analyze the amino acid composition of Stas2-15 protein. Using TMHMM2.0（ https://services.healthtech.dtu.dk/services/TMHMM-2.0/ ）Predicting the transmembrane domain of Stas2-15 protein; Using the online tool ProtScale（ https://www.expasy.org/ ）Analyze its hydrophilicity and hydrophobicity; Using the online tool SOPMA（ https://npsa-prabi.ibcp.fr/cgi-bin/npsa_automat.pl?pg=npsa_sopma.html ）Predicting secondary structure; Using the threading method I-TASSER（ https://www.aideepmed.com/I-TASSER/ ）Predict the three-level structure. (2) RNA extraction and detection Entrust Baimaike (Qingdao) Co., Ltd. to conduct transcriptome sequencing. Total RNA was extracted from the sample using a reagent kit method, and the concentration, purity (OD260/OD280), and integrity of RNA were detected using a Nanodrop 2000 spectrophotometer. And evaluate RNA integrity through LabChip GX to ensure that the samples meet the requirements for library sequencing. (3) Construction and sequencing of cDNA library After the RNA samples were tested and qualified by Nanodrop2000 (manufacturer: Thermo Fisher, model: Nanodrop2000) and LabChip GX (manufacturer: Platinum Elmer, model: LabChip GX Touch HT), Illumina high-throughput sequencing library was used to construct a model for cDNA library preparation. Firstly, Oligo (dT) magnetic beads were used to specifically enrich eukaryotic mRNA and fragment it to an appropriate length. Subsequently, fragmented mRNA was used as a template for reverse transcription synthesis of cDNA chains, which were purified and subjected to end repair, A-stail addition, and sequencing adapter ligation. The purified cDNA was enriched by PCR to obtain a cDNA library suitable for high-throughput sequencing. After passing the Qsep-400 test, the cDNA library was sequenced and evaluated for quality using the Illumina high-throughput sequencing platform, including low-quality base and adapter sequence filtering. Data analysis covers reference genome alignment, gene expression quantification, and differential expression screening. Strict quality control thresholds and standardized parameters are set for each analysis step to ensure traceability and repeatability of the entire process, namely "sample collection RNA extraction library construction sequencing quality control data analysis", a stable and reproducible transcriptome sequencing and data analysis platform  transcriptome; potato; Stas2-15 Duan Yonghong Song Huiyang Zhou Qimi Gao Luyao Mei Chao Zhang Yaoyao Bai Xiaodong Feng Ruiyun PUBLIC https://opendatacommons.org/licenses/by/1-0/ dataset Science Data Bank