2026-10-12T11:11:38Z https://www.scidb.cn/oai

10.57760/sciencedb.j00139.000ag 2026-08-31T09:20:14Z

2026-08-31 Dataset of SSU rDNA, ITS-5.8S, and LSU rDNA sequences of litostomatean ciliates doi:10.57760/sciencedb.j00139.000ag en The dataset comprised sequences from 29 species (32 populations) of the ciliate class Litostomatea, including 26 SSU rDNA, 30 ITS-5.8S, and 32 LSU rDNA sequences. For each species, either a single cell or a small number of conspecific cells were isolated and washed five times with 0.22 μm filtered in situ water. Genomic DNA was extracted using the DNeasy Blood & Tissue Kit (Qiagen, Germany), following the manufacturer's protocol with a modification in which one-quarter of the recommended reagent volumes was used. PCR amplification was performed using Q5® Hot Start High-Fidelity 2x Master Mix DNA Polymerase (New England BioLabs, USA) to minimize experimental errors. PCR products were verified via agarose gel electrophoresis and sequenced bidirectionally by Tsingke Biological Technology Company (Qingdao, China). Sequence fragments were assembled using Seqman (DNAStar).  Ciliates; Litostomatea; SSU rDNA; ITS-5.8S; LSU rDNA Yong CHI RESTRICTED dataset Science Data Bank