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    <identifier>10.57760/sciencedb.j00139.00203</identifier>
    <datestamp>2025-04-21T10:04:01Z</datestamp>
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  <dc:date>2025-04-21</dc:date>
  <dc:title>Single-cell RNA-seq provides insight into the underdeveloped immune system of germ-free mice</dc:title>
  <dc:identifier>doi:10.57760/sciencedb.j00139.00203</dc:identifier>
  <dc:language>en</dc:language>
  <dc:description>This RDS file contains processed single-cell RNA sequencing (scRNA-seq) data comparing immune cell populations from germ-free (GF) and specific-pathogen-free (SPF) mice. The dataset includes:Samples: Peripheral blood (PB) and bone marrow (BM) from GF and SPF miceCell Counts:Raw: 21,827 cells (PB) and 19,940 cells (BM)Quality-filtered: 18,344 high-quality cells (PB) and 16,537 high-quality cells (BM)Gene Coverage: Median 1,426 genes per cell (PB) and 1,391 genes per cell (BM)Cell Classifications: 18 major cell identities further divided into 25 subpopulationsAnnotation: Cells identified using established marker genes for blood cells</dc:description>
  <dc:subject>germ-free mice; microbiota; single-cell RNA sequencing; underdeveloped immune system</dc:subject>
  <dc:creator>Yifei SHENG</dc:creator>
  <dc:creator>Xiaodong FANG</dc:creator>
  <dc:rights>PUBLIC</dc:rights>
  <dc:rights>https://creativecommons.org/licenses/by-nc/4.0/</dc:rights>
  <dc:type>dataset</dc:type>
  <dc:relation>http://www.doi.org/10.24272/j.issn.2095-8137.2024.467</dc:relation>
  <dc:publisher>Science Data Bank</dc:publisher>
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