2026-10-15T02:22:25Z https://www.scidb.cn/oai

10.57760/sciencedb.j00139.00348 2026-07-20T16:17:08Z

2026-07-20 A RNA Sequencing of TestisTissues from Global Bmal1 Knockout and Wide Type Mice doi:10.57760/sciencedb.j00139.00348 en The objective of this study was to understand the effects of global knockout of Bmal1 on the testicular transcriptome. A total of 12 8-10-week-old mice were used. All mice were maintained on a C57BL/6J background and backcrossed for at least five to ten generations to ensure genetic homogeneity. The mice were individually housed under controlled environmental conditions (22 ± 1°C, 50–60% humidity) with ad libitum access to food and water. Prior to experiments, all animals were entrained to a 12-h light / 12-h dark cycle (lights on at 08:00, ZT0; lights off at 20:00, ZT12) for at least two weeks to synchronize the endogenous circadian rhythms with the photoperiod. At the ZT 0 and ZT 12 time points, three mice in each of the Bmal1-/- group and the control group were sacrificed, and their testicles were extracted. Testicular RNA was isolated and subjected to RNA-seq. Testis; Bmal1; mice Yating Li Guohao Han Xiaoyun Xiao Chao Li Hao Dong Wei Liu Hongcong Zhao Wanghao Yang Dan Li Haodong Xu Aihua Wang Yaping Jin Haisen Zhang Hsu-Wen Chao Huatao Chen PUBLIC https://creativecommons.org/licenses/by/4.0/ dataset Science Data Bank