2026-10-11T21:09:42Z https://www.scidb.cn/oai

10.57760/sciencedb.j00203.00061 2026-02-05T09:48:28Z

2026-02-05 LCMS_Raw_Data_NPPM_Radiosensitization_Study doi:10.57760/sciencedb.j00203.00061 en This dataset contains raw untargeted metabolomics data derived from human glioblastoma U87 MG cells, generated to investigate the radiosensitizing mechanism of biomimetic Fe3O4 nanoparticles (NPPM).Experimental Design and Sample Preparation:U87 MG cells were divided into four experimental groups: Negative Control (NC), NPPM Only (NP), Irradiation Only (IR), and NPPM combined with Irradiation (NP_IR). Cells in the NP and NP_IR groups were treated with NPPM (100 μmol/L). After 24 hours of drug incubation, the medium was replaced, and the IR and NP_IR groups received a single dose of 5 Gy radiation (6 MV, 600 cGy/min). Cells were harvested 24 hours post-irradiation. Metabolites were extracted using a cold methanol/water (4:1, v/v) solution, followed by grinding, ultrasonication, and centrifugation to obtain the supernatant.Data Acquisition:The metabolic profiles were acquired using a Thermo Scientific Vanquish Horizon UHPLC system coupled with a Thermo Scientific Exploris 240 mass spectrometer. Chromatographic separation was performed on a Waters ACQUITY UPLC HSS T3 column (100 mm × 2.1 mm, 1.8 µm) at 40 °C. The mass spectrometer operated in both positive and negative electrospray ionization (ESI) modes, with a scan range of m/z 70–1050.Data Structure and Format:The dataset consists of raw mass spectrometry files in .raw format (Thermo Scientific proprietary format). The data are organized into two main categories based on the ionization mode: Positive Mode (POS) and Negative Mode (NEG). Quality Control (QC) samples are included to monitor instrument stability.Potential Usage:This dataset can be processed using metabolomics software (e.g., Progenesis QI) for peak alignment, normalization, metabolite identification. Untargeted Metabolomics; Glioblastoma; Radiosensitization ; LC-MS/MS; Biomimetic nanoparticles 王亮 尹亮亮 苟巧 吉艳琴 EMBARGO https://creativecommons.org/licenses/by-nc-sa/4.0/ dataset Science Data Bank