2026-10-13T12:33:59Z https://www.scidb.cn/oai

10.57760/sciencedb.j00210.00094 2026-01-19T16:17:44Z

2026-01-19 The haplotype-resolved assembly of Centella asiatica and its annotation file doi:10.57760/sciencedb.j00210.00094 en Extract genomic DNA with CTAB method and PacBio Sequel II platform was used to construct 15 kb DNA SMRTbell library. The circular consensus sequencing (CCS) was the raw sequencing data of the library. Hi-C data was sequenced using the platform of Illumina MGISEQ-T7. SMRT Link (v9.0.0) and Fastp (v0.23.4) were used to generate CCS reads and clean Hi-C reads, respectively. Hifiasm (v0.19.9), Juicer (v2.13.07), 3d-DNA (v190716) and Juicerbox (v1.11.08) was used for genome assembly . GenomeThreader (v1.7.1), HISAT2 (v2.2.1), StringTie (v2.1.4), TransDecoder (v5.5.0), Trinity (v2.12.0), PASA (v2.5.2), Augustus (v3.3.3), and EVidenceModeler (v2.1.0) were used to predict gene structure annotation. Centella asiatica; haplotype-resolved assembly; gene structure annotation 汪超 PUBLIC https://creativecommons.org/licenses/by-nc-nd/4.0/ dataset http://www.doi.org/10.1016/j.cj.2026.02.003 Science Data Bank