2026-10-13T23:40:30Z https://www.scidb.cn/oai

10.57760/sciencedb.j00217.03980 2024-11-04T17:02:22Z

2024-11-04 Mechanism of Sishen Pill on Ulcerative Colitis based on Network Pharmacology and animal experiments doi:10.57760/sciencedb.j00217.03980 en Objective To explore the mechanism of action of Sishen Pill in the treatment of Ulcerative colitis (UC) through network pharmacology and animal experiments.Methods TCMSP and BATMAN-TCM databases were used to obtain the components and targets of Sishen Pill; GeneCards, OMIM, and PharmGKB databases obtain UC targets, and Cytoscape 3.8. 2 software builds the "Sishen Pill-Active Ingredient-Intersection Targets" interaction network and PPI network, and performs GO and KEGG enrichment analysis on the intersection targets, and then The core components are molecularly docked with their targets. A mouse model of UC with spleen and kidney yang deficiency was constructed, and Sishen Pill was given intervention treatment. The network pharmacological prediction was verified by WB and RT-qPCR experiments.Results The core components of Sishen Pill in the treatment of UC include quercetin, β-sitosterol, cholecalciferol, delphinium pigment, puerarin, etc. The core targets include IL-6, TP53, EGFR, AKT1, IL1β, etc., and mainly play a role through IL-17, PI3K-Akt, Toll-like receptor, TRP signaling pathway, etc. The core component has good binding performance to the core target; Sishen Pill can reduce the expression of EGFR and IL-6 and increase the expression of p53 in mouse colon tissue.Conclusion Sishen Pill can regulate EGFR, IL-6, p53 and other targets, regulate IL-17, PI3K-Akt, Toll-like receptors, and TRP signaling pathways, reduce inflammatory damage and treat UC. Sishen Pill; Ulcerative colitis; Network Pharmacology; Molecular docking; Experimental verification Xiao-Ling.LI Yu-Hong.WU Chun.LIU Hai-Long.LI Jin-Li.ZHANG Yong-Lin.LIANG PUBLIC https://creativecommons.org/licenses/by-nc-nd/4.0/ dataset Science Data Bank