2026-10-12T23:12:18Z https://www.scidb.cn/oai

10.57760/sciencedb.j00217.08640 2025-07-10T16:59:26Z

2025-07-10 The Effects of High-salt-diet on Humoral Immunity are Mediated through the secretion of IL-2 doi:10.57760/sciencedb.j00217.08640 en Objective Based on previous studies how high salt diet affects cellular immunity,, this study explored how high salt diet affects humoral immunity and its possible molecular mechanisms of action..Methods KLH/CFA mice were randomly divided into a control group and a high salt diet group. The effects of high salt diet on the activity, proliferation, function, and differentiation of T and B cells were examined by flow cytometry; In addition, splenic T and B cells isolated mice were cultured, activated, and induced for the generation of activated B cells under iTreg and LPS stimulation. We further analyzed their mechanism of action under high salt diet.Results ① A high salt diet significantly promoted the Tfh amplification and humoral immunity. ② High salt diet significantly enhanced IL-2 production and cell proliferation in CD4+ T cells. ③ High salt diet markedly increased the plasma cell differentiation and facilitated IL-2 secretion in vitro. To our knowledge, it is first time that a high salt diet regulates humoral immunity by modulating Tfh expression and affecting IL-2. This helps provide new insights into the prognosis and dietary guidance for clinical treatment and humoral immune related diseases. Follicular Helper T Cell; B Cell; CD4+T Cell; IL-2; High-salt-diet; Humoral Immunity Chen.Ye Xiaojiao.Wei Song Guo.Zheng PUBLIC https://creativecommons.org/licenses/by/4.0/ dataset Science Data Bank