2026-10-13T05:11:36Z https://www.scidb.cn/oai

10.57760/sciencedb.j00266.00013 2026-05-07T11:04:39Z

2026-05-07 High-throughput sequencing data of phyllosphere microorganisms doi:10.57760/sciencedb.j00266.00013 en The sampling site was located in a Euonymus japonicus green belt around the Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Sampling was conducted in June 2019. The samples consisted of E. japonicus leaves infected with powdery mildew and healthy, uninfected leaves, with three replicates each. High-throughput sequencing of phyllosphere microorganisms was performed on these six samples. All raw data has been uploaded. The genes sequenced were: (i) the V5-V6 regions of the 16S ribosomal RNA (16S rRNA) gene, (ii) the fungal internal transcribed spacer (ITS) region​​, and (iii) the ​​nitrogenase reductase (nifH) gene​​, using primers 799F/1115R, ITS1F/ITS2R, and polF/polR. Phyllosphere; powdery mildew; high-throughput sequencing; bacteria; fungi; nitrogen-fixing bacteria; Euonymus japonicus Jiayu Liu Zhihui Bai PUBLIC https://creativecommons.org/licenses/by-nc-nd/4.0/ dataset http://www.doi.org/10.1016/j.ecofro.2026.05.002 Science Data Bank