2026-10-12T02:10:03Z https://www.scidb.cn/oai

10.57760/sciencedb.nprd.000cv 2026-09-07T10:52:21Z

2026-09-07 Exploring the Mechanism of Gansui-Gancao Pair in Treating Malignant Ascites Based on Network Pharmacology and Animal Experiments - Raw Data doi:10.57760/sciencedb.nprd.000cv en This study aims to explore the mechanism of Kansui Radix-Glycyrrhizae Radix et Rhizoma (KR-GRR) herb pair on malignant ascites (MA) by using network pharmacology and animal experiment. Firstly, the active components of KR-GRR herb pair and the corresponding targets were screened from the TCMSP database. The common targets and signaling pathways of KR-GRR herb pair in treating MA were predicted by network pharmacology, and a protein-protein interaction network was constructed to form a visualized map of Chinese Tradition medicine-components-diseases-targets. GO and KEGG analyses were used to predict the underlying mechanism of KR-GRR herb pair in treating MA. Secondly, molecular docking was employed to obtain the binding ability of the active components of KR-GRR herb pair to the core targets. Finally, the MA mice model was developed using H22 liver cancer cells for the mechanism validation. As a result, network pharmacology results indicated that 13 core bioactive components and 51 potential targets of KR-GRR against MA, with key targets including serine/threonine-protein kinase 1 (AKT1), tumor necrosis factor (TNF), interleukin- 6 (IL-6), tumor protein 53 (TP53), IL-1β, and Matrix metallopeptidase 9 (MMP9). A total of 141 signaling pathways were screened out by KEGG analysis, and molecular docking results suggested that the PI3K/AKT1/MMP9 signaling pathway played a pivotal role in the therapeutic effects of KR-GRR against MA. The animal experiment demonstrated that KR-GRR herb pair significantly improved the general condition, survival rate, and peritoneal histopathological damage in MA mice. Additionally, 2.5 g/kg of KR-GRR reduced abdominal circumference and ascites volume, and suppressed the expression of AKT1 and MMP9 in ascitic fluid. Furthermore, KR-GRR downregulated the mRNA levels of AKT1, TNF, IL-6, TP53, IL-1β, and MMP9 in peritoneal tissues, as well as the protein expression of p-PI3K, PI3K, p-AKT1, AKT1, and MMP9, especially the ratios of p-PI3K/PI3K and p-AKT1/AKT1. Taken together, KR-GRR herb pair may exert therapeutic effects against MA by inhibiting the activation of the PI3K/AKT1/MMP9 signaling pathway. Network Pharmacology; Animal Experiments; Gansui-Gancao Pair; Malignant Ascites he tian tian RESTRICTED dataset Science Data Bank