2026-10-06T15:50:49Z https://www.scidb.cn/oai

10.57760/sciencedb.nprd.000dx 2026-09-30T14:59:38Z

2026-09-30 Exploring the Myocardial Protective Mechanism of Total Flavonoids of Apocynum venetum L. Based on Network Pharmacology and Animal Experiments doi:10.57760/sciencedb.nprd.000dx en Abstract: To investigate the mechanism of total flavonoids of Apocynum venetum L. (AVL) against myocardial ischemia-reperfusion injury (MIRI) in rats based on network pharmacology and animal experiments. Common targets of AVL total flavonoids and MIRI were obtained using the TCMSP, SwissTargetPrediction, and GenCards databases; a protein-protein interaction (PPI) network was constructed via the STRING database and Cytoscape 3.10.0 software; Gene Ontology (GO) functional annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed using the DAVID database; molecular docking was conducted with AutoDockTools. Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was performed for qualitative and quantitative analysis of the chemical constituents of AVL total flavonoids. The MIRI rat model was established and the animals were divided into Sham group, Model group, AVL total flavonoids low and high dose groups (AVLL, AVLH), and LY294002 + AVL total flavonoids high dose group (LY294002+AVLH). Lead II electrocardiograms were monitored; myocardial infarct size was measured by TTC staining; pathological morphological changes were observed by HE staining; cardiomyocyte apoptosis rate was evaluated by TUNEL staining; and related protein expression was detected by Western blot. UPLC-MS/MS chemical composition identification showed that a total of 72 flavonoid compounds were identified. three major active components(luteolin, catechin,and kaempferol) and 75 common targets were screened out by network pharmacology methods, among which eight proteins including protein kinase B1 (AKT1), prostaglandin-endoperoxide synthase 2 (PTGS2), SRC, B-cell lymphoma/leukemia 2 (BCL2), and matrix metallopeptidase 9 (MMP9) were identified as core targets; GO analysis indicated that the main targets are involved in apoptotic processes; while KEGG analysis showed significant enrichment of the phosphatidylinositol 3 kinase-protein kinase B (PI3K-AKT) signaling pathway (P < 0.05). Molecular docking demonstrated that the three active components all stably bound to PTGS2, SRC, BCL2, and MMP9 proteins (binding energies < −7 kcal/mol). Animal experiments showed that AVL total flavonoids significantly reduced myocardial infarct size (P < 0.05), ameliorated histopathological damage, and decreased the apoptotic rate (P < 0.05); downregulated the protein expression of PTGS2 and MMP9 (P < 0.05), while upregulated BCL2 and SRC protein expression (P < 0.05); and LY294002 reversed the effects of AVL total flavonoids on p-AKT and SRC. In conclusion, AVL total flavonoids may protect the myocardium by binding to SRC and thereby regulating the PI3K-AKT signaling pathway. Key words: Total flavonoids of Apocynum venetum L.; Myocardial ischemia-reperfusion injury; Network pharmacology; Animal experiments; Molecular docking Wang Yangyang EMBARGO https://creativecommons.org/licenses/by-nc-nd/4.0/ dataset Science Data Bank