2026-10-14T04:07:29Z https://www.scidb.cn/oai

10.57760/sciencedb.nprd.00195 2026-05-29T10:50:44Z

2026-05-29 Dataset for network Pharmacology-Based Study on the Skin Whitening Effect and Mechanism of the Combined Extract of Rosa rugosa, Rosa chinensis, and Paeonia suffruticosa doi:10.57760/sciencedb.nprd.00195 en This study systematically evaluated the whitening efficacy, active components, and mechanism of action of the combined extract of Rosa rugosa, Rosa chinensis, and Paeonia suffruticosa (RRPE). The results showed that RRPE significantly inhibited tyrosinase activity and melanin production in both B16F10 cells and zebrafish models. A total of 155 components were identified in RRPE using UPLC-Q-TOF-MS, and network pharmacology predicted that RRPE acts through the AKT-GSK3β axis. Further validation by RT-qPCR and Western blot demonstrated that RRPE downregulated the transcriptional and protein levels of core targets in the AKT-GSK3β/MITF-TYR signaling axis, activated AKT phosphorylation, and enhanced the inhibitory effect on GSK-3β. These findings reveal, at the molecular level, the multi-node mechanism by which this extract suppresses melanin production. Rosa rugosa-Rosa chinensis-Paeonia suffruticosa extract; Skin whitening; network pharmacology; experimental validation; AKT/GSK-3β pathway Guan Ning Yu Xiaoyi Huang Liqing Li Mingkun Yan Jiangang Yan Yongtao Ma Yanlong RESTRICTED dataset Science Data Bank